JAN 22 / 2020
BillionToOne, a venture-backed precision diagnostics company, is presenting a poster at 20th Advances in Genome Biology and Technology Conference about a novel method to quantify the abundance of nucleic acid that spans over several orders of magnitude while significantly reducing the number of required sequencing reads.
Dilution Tagging: A novel method to quantify the abundance of nucleic acid species with high dynamic range.
Nucleic acid (DNA and RNA) abundances in a single sample can span many orders of magnitude. In applications like characterization of T cell receptor population and RNA transcriptome measurements, quantifying molecules across many orders of magnitude, i.e., high dynamic range, is critical to understanding important developmental or disease states.
Current methods to quantify either DNA or RNA with high dynamic range, quantitative real-time PCR (qPCR) and unique molecular identifiers (UMIs), have limitations. BillionToOne developed a patent-pending, novel assay called dilution tagging that allows nucleic acid quantification over several orders of magnitude while significantly reducing the number of required sequencing reads.
In the poster presentation, we show that dilution tagging highly correlates with UMI based counting and is up to 62x more efficient than UMI-based counting.
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